Recent Reviews:
Brown, H.A. and Murphy, R.C. (2009) Working towards an exegesis for lipids in biology. Nature Chemical Biology Sept 5(9): 602-606.
Ivanova, P.T., Milne, S.B., Myers, D.S., and Brown, H.A. (2009) Lipidomics: a mass spectrometry based, systems level analysis of cellular lipids. Current Opinion in Chemical Biology. 2009 Dec13(5-6):526-31.
Brown HA. New mechanism for a classic folate inhibitor.(2008) Nature Chemical Biology Oct;4(10):581-2.
Ivanova, P.T., Milne, S.B., Byrne, M.O., Xiang, Y., and Brown, H.A. (2007) Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry. In Methods in Enzymology. 432, Lipidomics and Bioactive Lipids: Mass spectrometry based lipid analysis. Edited by H. Alex Brown. Elsevier. pp. 21-57.
Brown, H.A., Henage, L.G., Preininger, A.M., Xiang, Y., and Exton, J.H. (2007) Biochemical Analysis of Phospholipase D. In Methods in Enzymology. 434, Lipidomics and Bioactive Lipids: Lipids and Cell Signaling. Edited by H. Alex Brown. Elsevier. pp. 49-87.
Recent Publications By Year:
2010
Milne, S.B., Tallman, K.A., Serwa, R., Rouzer, C.A., Armstrong, M.A., Marnett, L.J., Lukehart, C.M., Porter, N.A., and Brown, H.A. (2010) Capture and release of Alkyne-Derivatized Glycerophospholipids using cobalt chemistry. Nature Chemical Biology. 2010 Mar;6(3):205-207. Epub 2010 Jan 24.
Shulga,Y.V., Myers, D.S., Ivanova, P.T., Milne, S.B., Brown, H.A., Topham, M.K., Epand, R.M. (2010) Molecular Species of Phosphatidylinositol-Cycle Intermediates in the Endoplasmic Reticulum and Plasma Membrane. Biochemistry Jan 19;49(2):312-7.
Callender, H.L., Horn, M.A., DeCamp, D.L., Sternweis, P.C., and Brown, H.A. (2010) Modeling Species-Specific Diacylglycerol Dynamics in the RAW 264.7 Macrophage. J. Theor. Biol. (in press).
Ivanova, P.T., Milne, S.B., and Brown, H.A. Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry. J. Lipid Res. 2009 Nov 30. [Epub ahead of print]
Rebres RA, Moon C, DeCamp D, Lin KM, Fraser ID, Milne SB, Roach TIA, Brown HA, Seaman WE. (2010) Clostridium difficile Toxin B differentially affects GPCR-stimulated Ca2+ responses in macrophages: independent roles for Rho and PLA2. J Leuko Biol. 2010 Mar 3. [Epub ahead of print].
2009
Scott SA, Selvy PE, Buck JR, Cho HP, Criswell TL, Thomas AL, Armstrong MD, Arteaga CL, Lindsley CW, Brown HA. Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness. Nature Chemical Biology 2009 Feb;5(2):108-17. Epub 2009 Jan 11.
Lewis JA, Scott SA, Lavieri R, Buck JR, Selvy PE, Stoops SL, Armstrong MD, Brown HA, Lindsley CW. Design and synthesis of isoform-selective phospholipase D (PLD) inhibitors. Part I: Impact of alternative halogenated privileged structures for PLD1 specificity. Bioorg Med Chem Lett. 2009 Apr 1;19(7):1916-20. Epub 2009 Feb 20. PMID: 19268584
Lavieri, R., Scott, S.A., Lewis, J.A., Selvy, P.E., Armstrong, M.D., Brown, H.A., Lindsley. C.W. Design and synthesis of isoform-selective phospholipase D (PLD) inhibitors. Part II. Identification of the 1,3,8-triazaspiro[4,5]decan-4-one privileged structure that engenders PLD2 selectivity. Bioorg Med Chem Lett. 2009 19: 2240–2243
Zeng, X., Zheng, X., Xiang, Y., Plumley, H., Jessen, J.R., Zhong, T.P., Solnica-Krezel, L., and Brown, H.A. Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish. Dev Biol. 2009 Apr 15;328(2):363-76. Epub 2009 Feb 4.
Rappley I, Gitler AD, Selvy PE, LaVoie MJ, Levy BD, Brown HA, Lindquist S, Selkoe DJ. Evidence that alpha-synuclein does not inhibit phospholipase D. Biochemistry. 2009 Feb 10;48(5):1077-83.
Rappley I, Myers DS, Milne SB, Ivanova PT, Lavoie MJ, Brown HA, Selkoe DJ. Lipidomic profiling in mouse brain reveals differences between ages and genders, with smaller changes associated with alpha-synuclein genotype. J Neurochem. 2009 Oct;111(1):15-25. Epub 2009 Jul 17.
Burnum KE, Cornett DS, Puolitaival SM, Milne SB, Myers DS, Tranguch S, Brown HA, Dey SK, Caprioli RM. Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation. J Lipid Res. 2009 May 8. [Epub ahead of print]
Holinstat M, Preininger AM, Milne SB, Hudson WJ, Brown HA, Hamm HE. Irreversible platelet activation requires protease-activated receptor 1-mediated signaling to phosphatidylinositol phosphates. Mol Pharmacol. 2009 Aug;76(2):301-13. Epub 2009 May 29.
Marlo JE, Niswender CM, Days EL, Bridges TM, Xiang Y, Rodriguez AL, Shirey JK, Brady AE, Nalywajko T, Luo Q, Austin CA, Williams MB, Kim K, Williams R, Orton D, Brown HA, Lindsley CW, Weaver CD, Conn PJ.(2009) Discovery and characterization of novel allosteric potentiators of M1 muscarinic receptors reveals multiple modes of activity.Mol Pharmacol. 2009 Mar;75(3):577-88.
Lung, M., Shulga, Y.V., Ivanova P.T., Myers, D.S., Milne, S.B., Brown, H.A., Topham, M.K., and Epand, R.M. (2009) Diacylglycerol Kinase ε Is Selective for Both Acyl Chains of Phosphatidic Acid or Diacylglycerol. J. Biol Chem. 2009 Nov 6;284(45):31062-73. Epub 2009 Sep 10.
2008
Garcia A, Zheng Y, Zhao C, Toschi A, Fan J, Shraibman N, Brown HA, Bar-Sagi D, Foster DA, Arbiser JL. (2008) Honokiol suppresses survival signals mediated by Ras-dependent phospholipase D activity in human cancer cells. Clin Cancer Res. 1;14(13):4267-74.
Sun Y, Fang Y, Yoon MS, Zhang C, Roccio M, Zwartkruis FJ, Armstrong M, Brown HA, Chen J. (2008) Phospholipase D1 is an effector of Rheb in the mTOR pathway.Proc Natl Acad Sci U S A. 105(24):8286-91.
Milne SB, Ivanova PT, Armstrong MD, Myers DS, Lubarda J, Shulga YV, Topham MK, Brown HA, Epand RM. Dramatic Differences in the Roles in Lipid Metabolism of Two Isoforms of Diacylglycerol Kinase. Biochemistry. 2008 Sep 9;47(36):9372-9. Epub 2008 Aug 15.
2007
Rouzer, C.A., Ivanova, P.T., Byrne, M.O., Brown, H.A., and Marnett, L.J., Lipid Profiling Reveals Glycerophospholipid Remodeling in Zymosan-Stimulated Macrophages. Biochemistry, 46:6026-6042.
Murphy, S.C, Fernandez-Pol, S., Chung, P.H., Murthy, S.N., Milne, S.B., Salomao, M., Brown, H.A., Lomasney, J.W., Mohandas, N., and Haldar, K. (2007) Cytoplasmic remodeling of erythrocyte raft lipids during infection by the human malaria parasite Plasmodium falciparum. Blood 15, 2132-2139.
Sud, Manish; Fahy, Eoin; Cotter, Dawn; Brown, Alex; Dennis, Edward; Glass, Christopher; Merrill, Al; Murphy, Robert; Raetz, Christian; Russell, David; Subramaniam, Shankar. (2007) LMSD: LIPID MAPS structure database. Nucleic Acids Research, Jan 35:D527-532.
Callender, H.L., Forrester, J.S., Ivanova, P., Preininger, A., Milne, S., and Brown, H.A., (2007) Quantification of diacylglycerol species from biological extracts by electrospray ionization mass spectrometry. Analytical Chemistry 79: 263-272.
2006
Milne, S.B., Ivanova, P.T., Forrester, J.S., and Brown, H.A., (2006) Lipidomics: Analysis of cellular lipids by ESI-MS. Methods 39: 92-103. Edited by V. Bankaitis. Elsevier Press.
Henage, L., Exton, J. and Brown, H.A. Kinetic analysis of a mammalian Phospholipase D: Kinetic analysis of a mammalian Phospholipase D: Allosteric modulation by monomeric GTPases, Protein kinase C and polyphosphoinosites.(2006) J. Biol. Chem. 281: 3408-3417.
Preininger, A.M., Henage, L., Oldham, W., Yoon, E.J., Hamm, H.E., and Brown, H.A., (2006) Direct modulation of phospholipase D activity by Gbg . Molecular Pharmacology 70:311-318.
Rouzer, R.A., Ivanova, P.T., Byrne, M.O., Milne, S.B., Marnett, L.J., and Brown, H.A. , (2006) Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications., Biochemistry 45: 14795-14808.
Milne, S.B., Forrester, J.S., Ivanova, P.T., Armstrong, M.D., and Brown, H.A., (2006) Multiplexed Lipid Arrays of Antiimmunoglobulin M-Induced Changes in the Glycerophospholipid Composition of WEHI-231 Cells In: Functional Lipidomics. edited by L. Feng and G. D. Prestwich. CRC Press, Boca Raton, FL.
Selected Publications From 2005 and Beyond
Dennis, E.A., Brown, H.A., Deems, R.A., Glass, C.K., Merrill, A.H., Murphy, R.C., Raetz, C.R., Shaw, W., Shankar, S., Russell, D.W., VanNieuwenhze, M.S., White, S.H., Witztum, J.L., and Wooley, J. (2006) The LIPID MAPS Approach To Lipidomics. In: Functional Lipidomics. edited by L. Feng and G. D. Prestwich. CRC Press, Boca Raton, FL..
Fahy, E., Subramaniam, S., Brown, H.A., Glass, C.K., Merrill, A.H., Murphy, R.C., Raetz, C.R., Russell, D.W., Seyama, Y., Shaw, W., Shimizu, T., Spener, F., van Meer, G., VanNieuwenhze, M.S., White, S.H., Witztum, J., and Dennis, E.A. (2005) A Comprehensive Classification System for Lipids. J. Lipid Research 46: 839-862.
Fang, Y., Park, I-H., Wu, A-L., Du, G., Huang, P., Frohman, M.A., Walker, S.J., Brown, H.A., Chen, J. (2003) PLD1 regulates mTOR signaling and mediates Cdc42 activation of S6K1. Current Biology13:2037-2044.
Forrester, J.S., Milne, S.B., Ivanova, P.T., and Brown, H.A. (2004) Computational Lipidomics: A multiplexed analysis of dynamic changes in membrane lipid composition during signal transduction. Molecular Pharmacology 65:813-821.
Ivanova, P.T., Milne, S.B., Forrester, J.S., and Brown, H.A. (2004) Lipid Arrays: New tools in the understanding of membrane dynamics and lipid signaling. Molecular Interventions 4:86-96.
Ge, M., Gidwani, A., Brown, H.A., Holowka, D., Baird, B., and Freed, J.H. (2003) Ordered and disordered phases coexist in plasma membrane vesicles of RBL-2H3 mast cells. An ESR Study. . Biophysical J. 85: 1278-88.
Walker, S.J. and Brown, H.A. (2003) Measurement of G protein stimulated phospholipase D activity in intact cells. In Methods in Molecular Biology vol 237: G Protein Signaling: Methods and Protocols. edited by A.V. Smrcka.Humana Press Inc., Totowa, NJ.
Walker, S.J. and Brown, H.A. (2002) Specificity of Rho insert-mediated activation of phospholipase D1. J. Biol Chem. 277:26260-267.
Eisen, S. and Brown, H.A. (2002) Selective estrogen receptor modulators differentially regulate PLD catalytic activity in ER-negative breast cancer cells. Molecular Pharmacology 62:911-920.
Gilman, A.G. et al., (2002) Overview of the alliance for cellular signaling. Nature 420: 703-706.
Sieczkarski, S.B., Brown, H.A., Whittaker, G.R. (2003) The role of protein kinase C bII in influenza virus entry via late endosomes. J. Virology 77: 460-469.
Ivanova, P.T., Cerda, B A., Horn, D.M., Cohen, J.S., McLafferty, F.W., and Brown, H.A. (2001) Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation. Proc. Natl. Acad. Sci. USA. 98(13): 7152-7157.
Cohen, J.S. and Brown, H.A. (2001) Phospholipases stimulate secretion in RBL mast cells. Biochemistry 40: 6589-6597.
Ge, M., Cohen, J.S., Brown, H.A., and Freed, J.H. (2001) ADP ribosylation factor 6 binding to Phosphatidylinositol 4,5-bisphosphate-containing vesicles creates defects in the bilayer structure: An ESR Study. Biophysical J. 81(2): 994-1005.
Walker, S.J., Wu, W. J., Cerione, R.A., and Brown, H.A. (2000) Activation of Phospholipase D1 by Cdc42 Requires the Rho Insert Region. J. Biol. Chem. 275: 15665-15668.
Brown, H.A. and Sternweis, P.C. (1995) Stimulation of phospholipase D by ADP-ribosylation factor. In Methods in Enzymology. Vol. 257. W.E. Balch, C.J. Der, and A. Hall, eds. Academic Press. pp. 313-324.
Ktistakis, N., Brown, H.A., Sternweis, P.C., and Roth, M.G. (1995) Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin A. Proc. Natl. Acad. Sci. USA 92: 4952-4956.
Brown, H.A., Gutowski, S., Kahn, R.A., and Sternweis, P.C. (1995) Partial purification and characterization of Arf-sensitive phospholipase D from porcine brain. J. Biol. Chem. 270: 14935-14943.
Singer, W.D., Brown, H.A., Bokoch, G.M., and Sternweis, P.C. (1995) Resolved phospholipase D activity is modulated by cytosolic factors other than Arf. J. Biol. Chem. 270: 14944-14950.
Brown, H.A., Gutowski, S., Moomaw, C.R., Slaughter, C., and Sternweis, P.C. (1993) ADP-ribosylation factor (ARF), a small GTP-dependent regulatory protein, stimulates phospholipase D activity. Cell 75: 1137-1144
