Recent Reviews:
Selvy PE, Lavieri RL, Lindsley CW, and Brown HA. (2011). Phospholipase D-enzymology, functionality, and chemical modulation. Chemical Reviews –Lipid Biochemistry, Metabolism and Signaling. (in press)
Myers DS, Ivanova PT, Milne SB, and Brown HA. (2011) Quantitative analysis of glycerophospholipids by LC-MS: acquisition, data handling and interpretation. Biochim Biophys Acta-Molecular and Cell Biology of Lipids Jun 12 Epub ahead of print PMID 21683157
Brown HA and Murphy RC. (2009) Working towards an exegesis for lipids in biology. Nature Chemical Biology 9: 602-606. PMID 19690530
Ivanova PT, Milne SB, Myers DS, and Brown HA. (2009) Lipidomics: a mass spectrometry based systems level analysis of cellular lipids. Current Opinion in Chemical Biology 13(5-6):526-531 PMC 2787871
Brown HA. (2008) New mechanism for a classic folate inhibitor Nature Chemical Biology 10:581-582 PMID 18800042
Ivanova PT, Milne SB, Byrne MO, Xiang Y, and Brown HA. (2007) Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry. In Methods in Enzymology 432, Lipidomics and Bioactive Lipids: Mass spectrometry based lipid analysis. Ed. by H. Alex Brown. Elsevier. pp. 21-57. PMID 17954212
Brown HA, Henage LG, Preininger AM, Xiang Y, and Exton JH. (2007) Biochemical Analysis of Phospholipase D. In Methods in Enzymology. 434, Lipidomics and Bioactive Lipids: Lipids and Cell Signaling. Ed. by H. Alex Brown. Elsevier. pp. 49-87. PMID17954242
2011
Lindsley CW and Brown HA. (2011) Phospholipase D as a therapeutic target in brain disorders. Neuropsychopharmacology Reviews (in press).
Moore J, Milne SB, Myers DS, Brown HA, and Shaw W (2011). Standardization and Quantification of Lysophosphatidic Acid Compounds by Normal Phase and Reversed Phase Chromatography – Tandem Mass Spectrometry. In Lysophospholipid Receptors: Signaling and Biochemistry. John Wiley & Sons, Inc.Ed. by Hla, Spiegel, Moolenaar, and Chun (in press).
Liu STH, Sharon-Friling R, Ivanova PT, Milne SB, Myers DS, Rabinowitz J, Brown HA, Shenk T. (2011) Synaptic vesicle-like lipidome of human cytomegalovirus virions reveals a role for SNARE machinery in virion egress. Proc Nat Acad Sci 108(31): 12869-12874. PMC 3150939
Gorden DL, Ivanova PT, Myers DS, McIntyre JO, VanSaun MN, Wright JK, Matrisian LM, and Brown HA. (2011) Increased diacylglycerols characterize hepatic lipid changes in progression of human nonalcoholic fatty liver disease; comparison to a murine model. PLoS ONE 6(8) : e22775.doi:10.1371/journal.pone.022775. PMC 3153459
Kang DW, Park MH, Lee YJ, Kim HS, Lindsley CW, Brown HA, Min DS. (2011) Autoregulation of phospholipase D activity is coupled to selective induction of phospholipaseD1 expression to promote invasion of breast cancer cells.Int. J. Cancer128(4): 805-816. PMID 20473892
Norton LJ, Zhang Q, Saqib KM, Schrewe H, Anderson KE, Lindsley CW, Brown HA, Simon A., Rudge SA, and Wakelam MJO. (2011) PLD1, rather than PLD2, regulates phorbol ester-, adhesion dependent-, and Fcγ receptor stimulated reactive oxygen species production in neutrophils. J. Cell Science 124(Pt.12): 1973-1983. PMC 3104032
2010
Milne SB, Tallman KA, Serwa R, Rouzer CA, Armstrong MA, Marnett LJ, Lukehart CM, Porter NA, and Brown HA. (2010) Capture and release of Alkyne-Derivatized Glycerophospholipids using cobalt chemistry. Nature Chemical Biology. 6(3):205-207. PMC 2822082
Shulga YV, Myers DS, Ivanova PT, Milne SB, Brown HA, Topham MK, Epand RM. (2010) Molecular Species of Phosphatidylinositol-Cycle Intermediates in the Endoplasmic Reticulum and Plasma Membrane. Biochemistry 49(2):312-317. PMC 2805767
Callender HL, Horn MA, DeCamp DL, Sternweis PC, and Brown HA. (2010) Modeling Species-Specific Diacylglycerol Dynamics in the RAW 264.7 Macrophage. J.Theor.Biol. 262(4):679-690. PMC 2822135
Ivanova PT, Milne SB, and Brown HA. (2010) Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry. J. Lipid Res. 51:1581-1590. PMC 3035522
Andreyev AY, Fahy E, Guan Z, Kelly S, Li X, McDonald JG, Milne S, Myers D, Park H, Ryan A, Thompson BM, Wang E, Zhao Y, Brown HA, Merrill A, Raetz CRH, Russell D, Subramaniam S, Dennis EA. (2010) Subcellular organelle lipidomics in TLR-4-activated macrophages. J. Lipid Res. 51:2785-2797. PMC 2918461
Quehenberger O, Armando AM, Brown HA, Milne SB, Myers DS, Merrill AH, Bandyopadhyay S., Jones KN, Kelly S, Shaner RL, Sullards CM, Wang E. Murphy RC, Barkley RM, Leiker TJ, Raetz CRH, Guan Z, Laird GM, Six DA, Russell DW, McDonald JG, Subramaniam S, Fahy E, Dennis EA. (2010) Lipidomics reveals remarkable diversity of lipids in human plasma. J. Lipid Res. 51:3299-3305. PMC 2952570
Klappe K, Dijkhuis A-J, Hummel I, van Dam A, Ivanova PT, Milne SB, Myers DS, Brown HA, Permentier H, Kok JW. (2010) Extensive sphingolipid depletion does not affect lipid raft integrity or lipid raft localization and efflux function of the ABC transporter MRP1. Biochem. J. 430:519-529. PMID 20604746
Lavieri R, Scott SA, Selvy PE, Kim K, Jadhav S, Morrison RD, Daniels S, Brown HA, Lindsley CW. (2010) Design, synthesis and biological evaluation of halogenated N-(2-(4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]decan-8-yl)ethylbenzamides: Discovery of an isoform selective small molecule phospholipase D2 (PLD2) inhibitor. J. Med. Chem. 53(18):6706-6719. PMID 20735042
Rebres RA, Moon C, DeCamp D, Lin KM, Fraser ID, Milne SB, Roach TIA, Brown HA, Seaman WE. (2010) Clostridium difficile Toxin B differentially affects GPCR-stimulated Ca2+ responses in macrophages: independent roles for Rho and PLA2. J. Leukoc Biol. 87(6):1041-1057. PMC 2872536
Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman G, Reichart D, Merrill AH Jr, Sullards MC, Wang E, Murphy RC, Raetz CR, Garrett TA, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. (2010) A mouse macrophage lipidome. J Biol Chem. 285(51):39976-39985. PMC 3000979
2009
Scott SA, Selvy PE, Buck JR, Cho HP, Criswell TL, Thomas AL, Armstrong MD, Arteaga CL, Lindsley CW, and Brown HA. (2009) Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness. Nature Chemical Biology 5(2): 108-117. PMID 19136975
Lewis JA, Scott SA, Lavieri R, Buck JR, Selvy PE, Stoops SL, Armstrong MD, Brown HA, Lindsley CW. (2009) Design and synthesis of isoform-selective phospholipase D (PLD) inhibitors. Part I. Impact of alternative halogenated privileged structures for PLD1 specificity. Bioorg Med Chem Lett. 19(7):1916-1920. PMID 19268584
Lavieri R, Scott SA, Lewis JA, Selvy PE, Armstrong MD, Brown HA, Lindsley CW. (2009) Design and synthesis of isoform-selective phospholipase D (PLD) inhibitors. Part II. Identification of the 1,3,8-triazaspiro[4,5]decan-4-one privileged structure that engenders PLD2 selectivity. Bioorg Med Chem Lett. 19: 2240–2243. PMID 19299128
Zeng XX, Zheng X, Xiang Y, Cho HP, Jessen JR, Zhong TP, Solnica-Krezel L, and Brown HA. (2009) Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish. Developmental Biology 328(2):363-376. PMC 2688691
Rappley I, Gitler AD, Selvy PE, LaVoie MJ, Levy BD, Brown HA, Lindquist S, Selkoe DJ. (2009) Evidence that alpha-synuclein does not inhibit phospholipase D. Biochemistry 48(5):1077-1083. PMC 2683767
Burnum KE, Cornett DS, Puolitaival SM, Milne SB, Myers DS, Tranguch S, Brown HA, Dey SK, Caprioli RM. (2009) Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation. J Lipid Res. 50(11):2290-2298 PMC 2759835
Holinstat M, Preininger AM, Milne SB, Hudson WJ, Brown HA, Hamm HE. (2009) Irreversible platelet activation requires protease-activated receptor 1-mediated signaling to phosphatidylinositol phosphates. Mol Pharmacol.76(2):301-313. PMC 2713123
Brown HA and Murphy RC. (2009) Working towards an exegesis for lipids in biology. Nature Chemical Biology5(9): 602-606. PMID 19690530
Ivanova PT, Milne SB, Myers DS, and Brown HA. (2009) Lipidomics: a mass spectrometry based systems level analysis of cellular lipids. Current Opinion in Chemical Biology 13:526-531. PMC 2787871
Rappley I, Myers DS, Milne SB, Ivanova PT, LaVoie MJ, Brown HA, Selkoe DJ. (2009) Lipidomics profiling in mouse brain reveals differences between ages and genders with smaller changes associated with α-synuclein genotype; J. Neurochem. 111(1): 15-25. PMC 2752313
Marlo JE, Niswender CM, Days EL, Bridges TM, Xiang Y, Rodriguez AL, Shirey JK, Brady AE, Nalywajko T, Luo Q, Austin CA, Williams MB, Kim K, Williams R, Orton D, Brown HA, Lindsley CW, Weaver CD, Conn PJ. (2009) Discovery and characterization of novel allosteric potentiators of M1 muscarinic receptors reveals multiple modes of activity. Mol Pharmacol. 75(3):577-588. PMC 2684909
Lung, M., Shulga, Y.V., Ivanova P.T., Myers, D.S., Milne, S.B., Brown, H.A., Topham, M.K., and Epand, R.M. (2009) Diacylglycerol Kinase Is Selective for Both Acyl Chains of Phosphatidic Acid or Diacylglycerol. J. Biol Chem 284(45):31062-31073. PMC 2781506
2008
Garcia A, Zheng Y, Zhao C, Toschi A, Fan J, Shraibman N, Brown HA, Bar-Sagi D, Foster DA, Arbiser JL. (2008) Honokiol suppresses survival signals mediated by Ras-dependent phospholipase D activity in human cancer cells. Clin Cancer Res. 1; 14(13):4267-4274. PMC 2759181
Sun Y, Fang Y, Yoon MS, Zhang C, Roccio M, Zwartkruis FJ, Armstrong M, Brown HA, Chen J. (2008) Phospholipase D1 is an effector of Rheb in the mTOR pathway. Proc Natl Acad Sci U S A. 105(24):8286-8291. PMC 2448829
Milne SB, Ivanova PT, Armstrong MD, Myers DS, Lubarda J, Shulga YV, Topham MK, Brown HA, Epand RM. (2008) Dramatic Differences in the Roles in Lipid Metabolism of Two Isoforms of Diacylglycerol Kinase. Biochemistry 47 (36):9372-9379. PMC 2744458
Brown HA. (2008) New mechanism for a classic folate inhibitor. Nature Chemical Biology 4(10): 581-582. PMID 18800042
2007
Sud M, Fahy E, Cotter D, Brown A, Dennis E, Glass CK, Merrill AH,Jr., Murphy RC, Raetz CR, Russell DW, Subramaniam S. (2007).LMSD: LIPID MAPS structure database. Nucleic Acids Research 35:D527-532. PMC 1669719
Callender HL, Forrester JS, Ivanova P, Preininger A, Milne S, and Brown HA. (2007) Quantification of diacylglycerol species from biological extracts by electrospray ionization mass spectrometry. Analytical Chemistry 79: 263-272. PMID 17194150
Rouzer CA, Ivanova PT, Byrne MO, Brown HA, and Marnett LJ. (2007) Lipid Profiling Reveals Glycerophospholipid Remodeling in Zymosan-Stimulated Macrophages. Biochemistry 46:6026-6042. PMID 17458939
Ivanova PT, Milne SB, Byrne MO, Xiang Y, and Brown HA. (2007) Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry. Methods Enzymol. 432, Lipidomics and Bioactive Lipids: Mass spectrometry based lipid analysis. Ed.by H. Alex Brown. Elsevier. pp. 21-57. PMID 17954212
Brown HA, Henage LG, Preininger AM, Xiang Y, and Exton JH. (2007) Biochemical Analysis of Phospholipase D. Methods Enzymol. 434, Lipidomics and Bioactive Lipids: Lipids and Cell Signaling. Ed. by H. Alex Brown. Elsevier. pp. 49-87. PMID 17954242
Murphy SC, Fernandez-Pol, S, Chung PH, Murthy SN, Milne SB, Salomao M, Brown HA, Lomasney JW, Mohandas N, and Haldar K. (2007) Cytoplasmic remodeling of erythrocyte raft lipids during infection by the human malaria parasite Plasmodium falciparum. Blood 15:2132-2139. PMC 1976375
2006
Dennis EA, Brown HA, Deems RA, Glass CK, Merrill AH, Murphy RC, Raetz CR, Shaw W, Subramaniam S, Russell DW, VanNieuwenhze MS, White SH, Witztum JL, and Wooley J. (2006) The LIPID MAPS Approach To Lipidomics. In Functional Lipidomics. Ed. by L. Feng and G. D. Prestwich. CRC Press, Boca Raton, FL.
Milne SB, Forrester JS, Ivanova PT, Armstrong MD, and Brown HA. (2006) Multiplexed Lipid Arrays of Antiimmunoglobulin M-Induced Changes in the Glycerophospholipid Composition of WEHI-231 Cells In: Functional Lipidomics. Ed. by L. Feng and G. D. Prestwich. CRC Press, Boca Raton, FL.
Henage L, Exton J, and Brown HA. (2006) Kinetic analysis of a mammalian Phospholipase D: Kinetic analysis of a mammalian Phospholipase D: Allosteric modulation by monomeric GTPases, Protein kinase C and polyphosphoinositides. J. Biol. Chem. 281: 3408-3417. PMID 16339153
Preininger AM Henage L, Oldham W, Yoon EJ, Hamm HE, and Brown HA. (2006) Direct modulation of phospholipase D activity by Gbg . Molecular Pharmacology70:311-318. PMID 16638972
Milne SB, Ivanova PT, Forrester JS, and Brown HA. (2006) Lipidomics: Analysis of cellular lipids by ESI-MS. Methods 39: 92-103.
Rouzer CA, Ivanova PT, Byrne MO, Milne SB, Marnett LJ, and Brown HA. (2006) Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications. Biochemistry 45: 14795-14808. PMC 2443946
Selected publications from 2005 and beyond
Brown HA, Gutowski S, Moomaw CR, Slaughter C, and Sternweis PC. (1993) ADP-ribosylation factor (ARF), a small GTP-dependent regulatory protein, stimulates phospholipase D activity. Cell 75: 1137-1144. PMID 8261513
Ktistakis N, Brown HA, Sternweis PC, and Roth MG. (1995) Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin A. Proc. Natl. Acad. Sci. USA 92: 4952-4956. PMID 7761430
Brown HA, Gutowski S, Kahn RA, and Sternweis PC. (1995) Partial purification and characterization of Arf-sensitive phospholipase D from porcine brain. J. Biol. Chem. 270: 14935-14943. PMID 7797473
Singer WD, Brown HA, Bokoch GM, and Sternweis PC. (1995) Resolved phospholipase D activity is modulated by cytosolic factors other than Arf. J. Biol. Chem. 270: 14944-14950. PMID 7797474
Brown HA and Sternweis PC. (1995) Stimulation of phospholipase D by ADP-ribosylation factor. In Methods in Enzymology 257. W.E. Balch, C.J. Der, and A. Hall, eds. Academic Press. pp. 313-324. PMID 8583935
Walker SJ, Wu W J, Cerione RA, and Brown HA. (2000) Activation of Phospholipase D1 by Cdc42 Requires the Rho Insert Region. J. Biol. Chem. 275: 15665-15668. PMID 10747870
Cohen JS and Brown HA. (2001) Phospholipases stimulate secretion in RBL mast cells. Biochemistry 40: 6589-6597. PMID 11380253
Ivanova PT, Cerda BA, Horn DM, Cohen JS, McLafferty FW, and Brown HA. (2001) Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation. Proc. Natl. Acad. Sci. USA. 98(13): 7152-7157. PMID 11416200
Ge M, Cohen JS, Brown HA, and Freed JH. (2001) ADP ribosylation factor 6 binding to Phosphatidylinositol 4,5-bisphosphate-containing vesicles creates defects in the bilayer structure: An ESR Study. Biophysical J. 81(2): 994-1005. PMID 11463641
Walker SJ and Brown HA. (2002) Specificity of Rho insert-mediated activation of phospholipase D1. J. Biol Chem. 277:26260-26267. PMID 12011045
Eisen S and Brown HA. (2002) Selective estrogen receptor modulators differentially regulate PLD catalytic activity in ER-negative breast cancer cells. Molecular Pharmacology 62:911-920. PMID 12237338
Gilman AG et al., (2002) Overview of the alliance for cellular signaling. Nature 420: 703-706. PMID 12478301
Sieczkarski SB, Brown HA, Whittaker GR. (2003) The role of protein kinase C bII in influenza virus entry via late endosomes. J. Virology 77: 460-469. PMID 12477851
Walker SJ and Brown HA. (2003) Measurement of G protein stimulated phospholipase D activity in intact cells. In Methods in Molecular Biology 237: G Protein Signaling: Methods and Protocols. Ed. by A.V. Smrcka. .Humana Press Inc., Totowa, NJ. PMID 14501041
Ge M, Gidwani A, Brown HA, Holowka D, Baird B, and Freed JH. (2003) Ordered and disordered phases coexist in plasma membrane vesicles of RBL-2H3 mast cells. An ESR Study. Biophysical J. 85: 1278-1288. PMID 1288567
Fang Y, Park I-H, Wu A-L, Du G, Huang P, Frohman MA, Walker SJ, Brown HA, Chen J. (2003) PLD1 regulates mTOR signaling and mediates Cdc42 activation of S6K1. Current Biology13:2037-2044. PMID 14653992
Forrester JS, Milne SB, Ivanova PT, and Brown HA. (2004) Computational Lipidomics: A multiplexed analysis of dynamic changes in membrane lipid composition during signal transduction. Molecular Pharmacology 65:813-821. PMID 15044609
Ivanova PT, Milne SB, Forrester JS, and Brown HA. (2004) Lipid Arrays: New tools in the understanding of membrane dynamics and lipid signaling. Molecular Interventions 4:86-96. PMID 15087482
Milne SB, Ivanova PT, DeCamp D, Hsueh RC, and Brown HA. (2005) A targeted mass spectrometric analysis of phosphatidylinositol phosphate species. J. Lipid Research 46: 1796-1802. PMID 15897608
Fahy E, Subramaniam S, Brown HA, Glass CK, Merrill AH, Murphy RC, Raetz CR, Russell DW, Seyama Y, Shaw W, Shimizu T, Spener F, van Meer G, VanNieuwenhze MS, White SH, Witztum J, and Dennis EA. (2005). A Comprehensive Classification System for Lipids. J. Lipid Research 46: 839-862. PMID 15722563
