Lee Henage , B.S.,
Emory University

Lee Henage is a pharmacology graduate student that initially trained in John Exton's laboratory. Lee is completing his dissertation research in the Brown Lab. Lee uses spectroscopic and computational methods to interrogate the structure of phospholipase D1. X-ray diffraction, magnetic resonance, and optical techniques are employed to address specific features of the enzyme's structure. PLD1 regulatory domains are examined by crystallographic methods while the catalytic core of the enzyme is probed using site-directed spin-labeling electron paramagnetic resonance methods (EPR). Existing structural information permits the construction of predictive molecular models of PLD1. These homology based models are refined with constraints derived from spectroscopic experiments. Refined three-dimensional models provide the first insight into the structural basis of PLD1 regulation and catalysis.